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货号: |
BI-20052 |
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方法学: |
三明治夹心法(ELISA) |
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样本类型: |
血清, EDTA血浆 |
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标准品范围: |
0-10 fmol/ml (6 serum based standards) |
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标准品浓度: |
0/0.625/1.25/2.5/5/10 fmol/ml |
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质控品: |
2个 |
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样本量: |
50 μl |
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孵育时间: |
overnight / 1 h / 30 min |
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单位换算: |
1 pg/ml = 0.4 fmol/ml (MW: 2.49 kDa) |
灵敏度:
LOD: 0.02 fmol/ml (0 fmol/ml + 3 SD)
精密度:
Intra-assay (n=18) ≤ 4%, Inter-assay (n=24) ≤ 6%
回收率:
The mean recovery of recombinant Endothelin (spike = 1 fmol/ml) is 96%.
The mean recovery of recombinant Endothelin (spike = 5 fmol/ml) is 86%.
交叉反应:
ET(1-21): 100%, ET2 (1-21): 100%, ET3 (1-21): <5%, Big Endothelin
(1-38): <1%, Big Endothelin ( 22-38): <1%.
In normal human plasma samples ET-2 is estimated to be present at less than 20%
of the ET-1 level. ET-3 is estimated to be present at 50% of the ET-1 level.
Animal sera: horse, cat, pig 100%; dog 66% and rat 79%, Mouse sera can not be
measured in this ELISA.
健康人群参考值:
Median (n=70): 0.26 fmol/ml.
推荐实验室建立自己的参考值。
本产品仅供科研使用